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KMID : 1234420120400030243
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Korean Journal of Microbiololgy and Biotechnology
2012 Volume.40 No. 3 p.243 ~ p.249
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Optimization of Conditions for the Production of Alginate-degrading Crude Enzyme from Vibrio crassostreae PKA 1002
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Sun Woo-Chan
Kim Koth-Bong-Woo-Ri
Kim Dong-Hyun
Jung Seul-A
Kim Hyun-Jee
Jeong Da-Hyun
Jeong Hee-Ye
Lim Sung-Mee
Hong Yong-Ki
Ahn Dong-Hyun
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Abstract
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This study was conducted to screen an alginate-degrading microorganism and to investigate the characteristics of the alginate-degrading activity of its crude enzyme. A marine bacterium which produces extracellular alginate-degrading enzymes was isolated from the brown alga Sargassum thunbergii. 16S rRNA sequence analysis and physiological profiling resulted in the bacterium’s identification as a Vibrio crassostreae strain, named Vibrio crassostreae PKA 1002. Its optimal culture conditions for growth were pH 9, 2% NaCl, 30oC and a 24 hr incubation time. The optimal conditions for the alginate degrading ability of the crude enzyme produced by V. crassostreae PKA 1002 were pH 9, 30oC, a 48 hr incubation time and 8% alginic acid. The alginate degrading crude enzyme produced 3.035 g of reducing sugar per liter in 4% (w/v) alginate over 1 hr.
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KEYWORD
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Alginate-degrading crude enzyme, Vibrio crassostreae PKA 1002
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